Purification and comparison of beta-1,3-glucan binding protein from white shrimp (Penaeus vannamei)

被引：48

作者：

VargasAlbores, F ^{[1
]}

JimenezVega, F ^{[1
]}

YepizPlascencia, GM ^{[1
]}

机构：

[1] BIOL RES CTR, LA PAZ 23000, MEXICO

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1997年 / 116卷 / 04期

关键词：

beta-1,3-glucan binding protein; BGBP; prophenoloxidase system; shrimp immunology; Penaeus vannamei; Penaeus stylirostris; PROPHENOLOXIDASE ACTIVATING SYSTEM; CRAYFISH PACIFASTACUS-LENIUSCULUS; CELLULAR COMMUNICATION; BLOOD-CELLS; HEMOLYMPH; ARTHROPODS; PLASMA; CALIFORNIENSIS; HEMAGGLUTININ; STYLIROSTRIS;

D O I：

10.1016/S0305-0491(96)00268-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A beta-glucan binding protein (BGBP) was identified in both white (Penaeus vannamei) and blue shrimp (P. stylirostris) plasma. White shrimp BGBP was purified by affinity chromatography using immobilized laminarin, and its molecular and biological properties were described. White shrimp BGBP is a monomeric protein with a molecular mass of 100 kDa, similar to those described for other crustacean BGBPs. White and blue shrimp BGBPs can be detected with antisera against crayfish BGBP and brown shrimp BGBP. Both amino acid composition and N-terminal sequence are markedly similar to brown shrimp (P. californiensis) and crayfish (Pacifastacus leniusculus) BGBP, indicating chat this recognition protein is present in freshwater and marine crustaceans. (C) 1997 Elsevier Science Inc.

引用

页码：453 / 458

页数：6

共 32 条

[1] PURIFICATION OF PROPHENOLOXIDASE FROM CRAYFISH BLOOD-CELLS, AND ITS ACTIVATION BY AN ENDOGENOUS SERINE PROTEINASE [J].