Simultaneous detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, three major fish pathogens, by multiplex PCR

被引：81

作者：

del Cerro, A

Marquez, I

Guijarro, JA ^{[1
]}

机构：

[1] Univ Oviedo, Area Microbiol, Dept Biol Func, Fac Med, E-33006 Oviedo, Spain

[2] SERIDA, Lab Sanidad Anim Jove, Gijon 33299, Asturias, Spain

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2002年 / 68卷 / 10期

关键词：

D O I：

10.1128/AEM.68.10.5177-5180.2002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A multiplex PCR assay based on the 16S rRNA genes was developed for the simultaneous detection of three major fish pathogens, Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri. The assay proved to be specific and as sensitive as each single PCR assay, with detection limits in the range of 6, 0.6, and 27 CFU for A. salmonicida, F. psychrophilum, and Y. ruckeri, respectively. The assay was useful for the detection of the bacteria in artificially infected fish as well as in fish farm outbreaks. Results revealed that this multiplex PCR system permits a specific, sensitive, reproducible, and rapid method for the routine laboratory diagnosis of infections produced by these three bacteria.

引用

页码：5177 / 5180

页数：4

共 22 条

[1] Detection of microbial pathogens in shellfish with multiplex PCR [J].