Point mutations in transmembrane helices 2 and 3 of ExbB and TolQ affect their activities in Escherichia coli K-12

被引：14

作者：

Braun, V ^{[1
]}

Herrmann, C ^{[1
]}

机构：

[1] Univ Tubingen, D-72076 Tubingen, Germany

来源：

JOURNAL OF BACTERIOLOGY | 2004年 / 186卷 / 13期

关键词：

D O I：

10.1128/JB.186.13.4402-4406.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Replacement of glutamate 176, the only charged amino acid in the third transmembrane helix of ExbB, with allanine (E176A) abolished ExbB activity in all determined ExbB-dependent functions of Escherichia coli. Combination of the mutations T148A in the second transmembrane helix and T181A in the third transmembrane helix, proposed to form part of a proton pathway through ExbB, also resulted in inactive ExbB. E176 and T148 are strictly conserved in ExbB and TolQ proteins, and T181 is almost strictly conserved in ExbB, TolQ, and MotA.

引用

收藏

页码：4402 / 4406

页数：5

相关论文

共 29 条

[1]

BLAIR DF, 1991, J MOL BIOL, V221, P1433, DOI 10.1016/0022-2836(91)90943-Z

[2] THE MOTA PROTEIN OF ESCHERICHIA-COLI IS A PROTON-CONDUCTING COMPONENT OF THE FLAGELLAR MOTOR [J].

BLAIR, DF ;

BERG, HC .

CELL, 1990, 60 (03) :439-449

[3] Flagellar movement driven by proton translocation [J].

Blair, DF .

FEBS LETTERS, 2003, 545 (01) :86-95

[4] THE PROTON MOTIVE FORCE DRIVES THE OUTER-MEMBRANE TRANSPORT OF COBALAMIN IN ESCHERICHIA-COLI [J].

BRADBEER, C .

JOURNAL OF BACTERIOLOGY, 1993, 175 (10) :3146-3150

[5] THE STRUCTURALLY RELATED EXBB AND TOLQ GENES ARE INTERCHANGEABLE IN CONFERRING TONB-DEPENDENT COLICIN, BACTERIOPHAGE, AND ALBOMYCIN SENSITIVITY [J].

BRAUN, V .

JOURNAL OF BACTERIOLOGY, 1989, 171 (11) :6387-6390

[6] Energy-coupled transport across the outer membrane of Escherichia coli: ExbB binds ExbD and TonB in vitro, and leucine 132 in the periplasmic region and aspartate 25 in the transmembrane region are important for ExbD activity [J].

Braun, V ;

Gaisser, S ;

Herrmann, C ;

Kampfenkel, K ;

Killmann, H ;

Traub, I .

JOURNAL OF BACTERIOLOGY, 1996, 178 (10) :2836-2845

[7]

BRAUN V, 1995, FEMS MICROBIOL REV, V16, P295, DOI 10.1016/0168-6445(95)00003-U

[8] EVOLUTIONARY RELATIONSHIP OF UPTAKE SYSTEMS FOR BIOPOLYMERS IN ESCHERICHIA-COLI - CROSS-COMPLEMENTATION BETWEEN THE TONB EXBB EXBD AND THE TOLA TOLQ TOLR PROTEINS [J].

BRAUN, V ;

HERRMANN, C .

MOLECULAR MICROBIOLOGY, 1993, 8 (02) :261-268

[9] Ton-dependent colicins and microcins: modular design and evolution [J].

Braun, V ;

Patzer, SI ;

Hantke, K .

BIOCHIMIE, 2002, 84 (5-6) :365-380

[10] The ToIQ-ToIR proteins energize ToIA and share homologies with the flagellar motor proteins MotA-MotB [J].

Cascales, E ;

Lloubès, R ;

Sturgis, JN .

MOLECULAR MICROBIOLOGY, 2001, 42 (03) :795-807