Architecture of the Trypanosoma brucei nucleus during interphase and mitosis

被引：114

作者：

Ogbadoyi, E ^{[1
]}

Ersfeld, K ^{[1
]}

Robinson, D ^{[1
]}

Sherwin, T ^{[1
]}

Gull, K ^{[1
]}

机构：

[1] Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England

来源：

CHROMOSOMA | 2000年 / 108卷 / 08期

基金：

英国惠康基金;

关键词：

D O I：

10.1007/s004120050402

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structural basis of mitosis, spindle organisation and chromosome segregation, in the unicellular parasite Trypanosoma brucei is poorly understood. Here, using immunocytochemistry, fluorescent in situ hybridisation and electron microscopy, we provide a detailed analysis of mitosis in this parasite. We describe the organisation of the mitotic spindle during different stages of mitosis, the complex ultrastructure of kinetochores and the identification of a potential spindle-organising centre in the mitotic nucleus. We investigate the dynamics of chromosome segregation using telomeric and chromosome-specific probes. We also discuss the problems involved in chromosome segregation in the light of the fact that the brucei karyotype has 22 chromosomes in the apparent presence of only eight ultrastructurally defined kinetochores.

引用

页码：501 / 513

页数：13

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