High-speed 1-frame/ms scanning confocal microscope with a microlens and Nipkow disks

被引：166

作者：

Tanaami, T

Otsuki, S

Tomosada, N

Kosugi, Y

Shimizu, M

Ishida, H

机构：

[1] Yokogawa Elect Corp, Musashino, Tokyo 1808750, Japan

[2] Tokai Univ, Sch Med, Dept Physiol, Isehara, Kanagawa 2591193, Japan

来源：

APPLIED OPTICS | 2002年 / 41卷 / 22期

关键词：

D O I：

10.1364/AO.41.004704

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We have developed a high-speed confocal laser microscope. A microlens-array disk set in front of a pinhole-array disk improved optical efficiency more than ten times compared with that of conventional Nipkow confocal microscopy. This new microscope achieves a high-speed measurement of 1 frame/ms. We expect that it will be used for measuring biological and industrial active samples. (C) 2002 Optical Society of America.

引用

页码：4704 / 4708

页数：5

共 4 条

[1] Visualization of biphasic Ca2+ diffusion from cytosol to nucleus in contracting adult rat cardiac myocytes with an ultra-fast confocal imaging system [J].