Insulin acts as a myogenic differentiation signal for neural stem cells with multilineage differentiation potential

被引:13
作者
Bani-Yaghoub, M
Kendall, SE
Moore, DP
Bellum, S
Cowling, RA
Nikopoulos, GN
Kubu, CJ
Vary, C
Verdi, JM
机构
[1] John P Robarts Res Inst, London, ON N6A 5K8, Canada
[2] Inst Res, Maine Med Ctr, Ctr Regenerat Med, Scarborough, ME 04074 USA
[3] Inst Res, Maine Med Ctr, Ctr Mol Med, Scarborough, ME 04074 USA
来源
DEVELOPMENT | 2004年 / 131卷 / 17期
关键词
plasticity; neural stem cells; myogenesis;
D O I
10.1242/dev.01295
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Reports of non-neural differentiation of neural stem cells (NSCs) have been challenged by alternative explanations for expanded differentiation potentials. In an attempt to demonstrate the plasticity of NSC, neurospheres were generated from single retrovirally labeled embryonic cortical precursors. In a defined serum-free insulin-containing media, 40% of the neurospheres contained both myogenic and neurogenic differentiated progeny. The number of NSCs displaying multilineage differentiation potential declines through gestation but does exist in the adult animal. In this system, insulin appears to function as a survival and dose-dependent myogenic differentiation signal for multilineage NSCs (MLNSC). MLNSC-derived cardiomyocytes contract synchronously, respond to sympathetic and parasympathetic stimulation, and regenerate injured heart tissues. These studies provide support for the hypothesis that MLNSCs exist throughout the lifetime of the animal, and potentially provide a population of stem cells for cell-based regenerative medicine strategies inside and outside of the nervous system.
引用
收藏
页码:4287 / 4298
页数:12
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