Icariin Treatment Rescues Diabetes Induced Bone Loss via Scavenging ROS and Activating Primary Cilia/Gli2/Osteocalcin Signaling Pathway

被引：21

作者：

Liu, Jie ^{[1
]}

Cheng, Qingfeng ^{[2
]}

Wu, Xiangmei ^{[3
]}

Zhu, Huifang ^{[1
]}

Deng, Xiaoyan ^{[1
]}

Wang, Maorong ^{[4
]}

Yang, Shengyong ^{[1
]}

Xu, Jie ^{[1
]}

Chen, Qian ^{[1
]}

Li, Mengxue ^{[1
]}

Liu, Xianjun ^{[1
]}

Wang, Changdong ^{[1
]}

机构：

[1] Chongqing Med Univ, Coll Basic Med, Mol Med & Canc Res Ctr, Dept Biochem & Mol Biol, Chongqing 400016, Peoples R China

[2] Chongqing Med Univ, Dept Endocrinol, Affiliated Hosp 1, Chongqing 401331, Peoples R China

[3] Chongqing Med Univ, Coll Basic Med, Mol Med & Canc Res Ctr, Dept Physiol, Chongqing 400016, Peoples R China

[4] Hubei Univ Nationalities, Dept Endocrinol, Affiliated Hosp, Enshi 445000, Peoples R China

来源：

CELLS | 2022年 / 11卷 / 24期

关键词：

diabetes; bone loss; primary cilia; Icariin; ROS; PRIMARY CILIA; RISK;

D O I：

10.3390/cells11244091

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Diabetes-associated bone complications lead to fragile bone mechanical strength and osteoporosis, aggravating the disease burden of patients. Advanced evidence shows that chronic hyperglycemia and metabolic intermediates, such as inflammatory factor, reactive oxygen species (ROS), and advanced glycation end products (AGEs), are regarded as dominant hazardous factors of bone complications, whereas the pathophysiological mechanisms are complex and controversial. By establishing a diabetic Sprague-Dawley (SD) rat model and diabetic bone loss cell model in vitro, we confirmed that diabetes impaired primary cilia and led to bone loss, while adding Icariin (ICA) could relieve the inhibitions. Mechanistically, ICA could scavenge ROS to maintain the mitochondrial and primary cilia homeostasis of osteoblasts. Intact primary cilia acted as anchoring and modifying sites of Gli2, thereby activating the primary cilia/Gli2/osteocalcin signaling pathway to promote osteoblast differentiation. All results suggest that ICA has potential as a therapeutic drug targeting bone loss induced by diabetes.

引用

页数：20

共 72 条

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