Populations of the south-west Pacific malaria vector Anopheles farauti s.s. revealed by ribosomal DNA transcribed spacer polymorphisms

被引:38
作者
Beebe, NW [1 ]
Cooper, RD
Foley, DH
Ellis, JT
机构
[1] Univ Technol Sydney, Dept Cell & Mol Biol, Mol Parasitol Lab, Sydney, NSW 2065, Australia
[2] Australian Army Malaria Inst, Enoggera, Qld 4052, Australia
[3] Univ Queensland, Trop Hlth Programme, St Lucia, Qld 4072, Australia
[4] Univ Queensland, Dept Zool & Entomol, St Lucia, Qld 4072, Australia
基金
澳大利亚研究理事会;
关键词
Anopheles farauti; concerted evolution; ITS2; malaria; mosquito; rDNA;
D O I
10.1046/j.1365-2540.2000.00665.x
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Malaria in the south-west Pacific is transmitted by members of the Anopheles punctulatus group which comprises 12 cryptic species with overlapping morphology. The most widely distributed species of the group is Anopheles farauti s.s. (An. farauti 1) found throughout northern Australia, Papua New Guinea, eastern Indonesia, the Solomon Islands and Vanuatu. A study of the population structure of this species using PCR-RFLP analysis on the ribosomal DNA internal transcribed spacer 1 reveals five genotypes which had distinct geographical distributions. Where these distributions overlap, genotype hybrids can be identified. Heteroduplex analysis of the ITS2 region reveals combinations of nonhomogenized ITS2 sequences and subsequently seven identifiable genotypes, reflecting the ITS1 distribution. Sequence analysis of these ITS2 polymorphisms reveals a minimum of 13 ITS2 sequence types present in heterogeneous combinations in individual mosquitoes. It appears that there are different levels of evolution occurring within the ITS1 and ITS2 regions. These data suggest that An. farauti s.s. may contain multiple loci for the rDNA gene family or that the homogenization of these regions is relatively slow and can be used in genetic studies of population distribution and structure.
引用
收藏
页码:244 / 253
页数:10
相关论文
共 31 条
[1]  
[Anonymous], 1983, Climate of Papua New Guinea
[2]   SPECIES COMPOSITION AND INVERSION POLYMORPHISM OF THE ANOPHELES-GAMBIAE COMPLEX IN SOME SITES OF GHANA, WEST-AFRICA [J].
APPAWU, MA ;
BAFFOEWILMOT, A ;
AFARI, EA ;
NKRUMAH, FK ;
PETRARCA, V .
ACTA TROPICA, 1994, 56 (01) :15-23
[3]   DNA sequence analysis of the ribosomal DNA ITS2 region for the Anopheles punctulatus group of mosquitoes [J].
Beebe, NW ;
Ellis, JT ;
Cooper, RD ;
Saul, A .
INSECT MOLECULAR BIOLOGY, 1999, 8 (03) :381-390
[4]   DISCRIMINATION OF ALL MEMBERS OF THE ANOPHELESPUNCTULATUS COMPLEX BY POLYMERASE CHAIN-REACTION RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM ANALYSIS [J].
BEEBE, NW ;
SAUL, A .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1995, 53 (05) :478-481
[5]   ANOPHELINE MOSQUITOES OF THE SOLOMON-ISLANDS AND NEW-HEBRIDES [J].
BELKIN, JN ;
KNIGHT, KL ;
ROZEBOOM, LE .
JOURNAL OF PARASITOLOGY, 1945, 31 (04) :241-265
[6]   BLOOD-FEEDING AND RESTING BEHAVIOR IN THE ANOPHELES-PUNCTULATUS DONITZ COMPLEX (DIPTERA, CULLICIDAE) FROM COASTAL PAPUA-NEW-GUINEA [J].
CHARLWOOD, JD ;
DAGORO, H ;
PARU, R .
BULLETIN OF ENTOMOLOGICAL RESEARCH, 1985, 75 (03) :463-475
[7]   A review of the use of ribosomal DNA (rDNA) to differentiate among cryptic Anopheles species [J].
Collins, FH ;
Paskewitz, SM .
INSECT MOLECULAR BIOLOGY, 1996, 5 (01) :1-9
[8]   A RIBOSOMAL-RNA GENE PROBE DIFFERENTIATES MEMBER SPECIES OF THE ANOPHELES-GAMBIAE COMPLEX [J].
COLLINS, FH ;
MENDEZ, MA ;
RASMUSSEN, MO ;
MEHAFFEY, PC ;
BESANSKY, NJ ;
FINNERTY, V .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1987, 37 (01) :37-41
[9]   CHROMOSOMAL DIFFERENTIATION AND ADAPTATION TO HUMAN ENVIRONMENTS IN THE ANOPHELES-GAMBIAE COMPLEX [J].
COLUZZI, M ;
SABATINI, A ;
PETRARCA, V ;
DIDECO, MA .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1979, 73 (05) :483-497
[10]  
Cooper RD, 1997, J AM MOSQUITO CONTR, V13, P5