CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes

被引：658

作者：

Komor, Alexis C. ^{[1
,2
,3
]}

Badran, Ahmed H. ^{[1
,2
,3
]}

Liu, David R. ^{[1
,2
,3
]}

机构：

[1] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

[2] Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA

[3] Broad Inst MIT & Harvard, Cambridge, MA 02141 USA

来源：

CELL | 2017年 / 168卷 / 1-2期

基金：

美国国家科学基金会;

关键词：

PLURIPOTENT STEM-CELLS; ZINC-FINGER NUCLEASES; DOUBLE-STRAND BREAKS; OFF-TARGET CLEAVAGE; DNA-CLEAVAGE; HOMOLOGOUS RECOMBINATION; KNOCK-IN; GENE DELIVERY; CAS9; PROTEIN; MOUSE MODEL;

D O I：

10.1016/j.cell.2016.10.044

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of advances in the life sciences that have grown from the ability to edit genomes within living cells. In this Review, we summarize CRISPR-based technologies that enable mammalian genome editing and their various applications. We describe recent developments that extend the generality, DNA specificity, product selectivity, and fundamental capabilities of natural CRISPR systems, and we highlight some of the remarkable advancements in basic research, biotechnology, and therapeutics science that these developments have facilitated.

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收藏

页码：20 / 36

页数：17

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