Partial purification of phytase from a soil isolate bacterium, Klebsiella oxytoca MO-3

被引：30

作者：

Jareonkitmongkol, S ^{[1
]}

Ohya, M ^{[1
]}

Watanabe, R ^{[1
]}

Takagi, H ^{[1
]}

Nakamori, S ^{[1
]}

机构：

[1] KYOTO PREFECTURAL UNIV,FAC BIOSCI,DEPT BIOSCI,MATSUOKA,FUKUI 91011,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1997年 / 83卷 / 04期

关键词：

Klebsiella oxytoca; phytase; phytic acid;

D O I：

10.1016/S0922-338X(97)80149-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A bacterial strain, Klebsiella oxytoca MO-3, producing phytase (EC 3.1.3.8) was isolated from compost. The phytase was extracted and purified 400-fold and partially characterized. The pH optimum was found to be in the range of 5-6 and the optimum temperature to be 55 degrees C. The phytase is stable at pH 4-8 and no loss of activity upon its incubation at 60 degrees C for 20 min was detected. The partially purified enzyme was strongly inhibited by NaF, Zn2+, Fe2+, and Cu2+ but not inhibited by EDTA or N-ethylmaleimide.

引用

页码：393 / 394

页数：2

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