Nutrient consumption and alkaloid accumulation in a hairy root line of Catharanthus roseus

被引：12

作者：

Moreno-Valenzuela, O ^{[1
]}

Coello-Coello, J ^{[1
]}

Loyola-Vargas, VM ^{[1
]}

Vázquez-Flota, F ^{[1
]}

机构：

[1] Ctr Invest Cient Yucatan, Unidad Biol Expt, Cordemex 97310, Yucatan, Mexico

来源：

BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 11期

关键词：

ajmalicine; bioreactor; catharanthine; Catharanthus roseus; hairy roots;

D O I：

10.1023/A:1005606818744

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

A hairy root line from Catharanthus roseus was cultured in a 14 l bioreactor. Nitrate and phosphate uptakes were similar to the same root line cultured in a 250 ml Erlenmeyer flask. However, sucrose consumption rate was slower in roots cultured in the bioreactor. These results show that it is feasible to upgrade this hairy root line to bioreactor level and, although carbon utilization has to be improved, ajmalicine and catharanthine were still produced and were retained within the biomass tissue.

引用

页码：1017 / 1021

页数：5

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