Effect of INT1 gene on Candida albicans murine intestinal colonization

被引:34
作者
Kinneberg, KM
Bendel, CM
Jechorek, RP
Cebelinski, EA
Gale, CA
Berman, JG
Erlandsen, SL
Hostetter, MK
Wells, CL
机构
[1] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA
[2] Univ Minnesota, Dept Pediat, Minneapolis, MN 55455 USA
[3] Univ Minnesota, Dept Plant Biol & Genet, Minneapolis, MN 55455 USA
[4] Univ Minnesota, Dept Genet, Minneapolis, MN 55455 USA
[5] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA
[6] Univ Minnesota, Dept Surg, Minneapolis, MN 55455 USA
[7] Yale Univ, Sch Med, Dept Pediat, New Haven, CT 06520 USA
关键词
Candida albicans; INT1; intestinal colonization; murine;
D O I
10.1006/jsre.1999.5755
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background. Increased intestinal colonization with Candida albicans is believed to be a major factor predisposing immunocompromised and postsurgical patients to systemic candidiasis, although the mechanisms facilitating C, albicans colonization remain unclear. Because previous studies have linked the C. albicans INT1 gene to filament formation, epithelial adherence, and mouse virulence, experiments were designed to evaluate the effect of INT1 on intestinal colonization. Materials and Methods, Mice were orally inoculated with either the parent strain (CAF2, INT/INT1), an int1 heterozygote (CAG1, INT1/int1), an int1 homozygote (CAG3, int1/int1), or a reintegrant (CAG5, int1/int1 + INT1), and sacrificed 3 and 7 days later for quantitative analysis of cecal C. albicans, Results. Following oral inoculation with 10(3) C. albicans, only small numbers of each strain were recovered from the cecal flora of normal mice. However, in mice pretreated with oral antibiotics, cecal colonization of each strain was increased (P < 0.01), In addition, cecal colonization was reduced for all int1 mutant strains compared with the parent strain (P < 0.05), By light microscopy, all four C. albicans strains were easily observed in the ileal lumen as both budding yeast and filamentous forms, although only occasional yeast forms appeared adherent to the intestinal epithelium. Conclusions, C. albicans readily colonized and replicated in the ceca of antibiotic-treated mice. The presence of two functional copies of INT1 appeared to facilitate C. albicans cecal colonization, suggesting that intestinal colonization may be another virulence factor associated with INT1 and that the gene product may be an attractive target to control C. albicans intestinal colonization. (C) 1999 Academic Press.
引用
收藏
页码:245 / 251
页数:7
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