The ParaSight(TM)-F rapid dipstick antigen capture assay for monitoring parasite clearance after drug treatment of Plasmodium falciparum malaria

被引:18
作者
DiPerri, G
Olliaro, P
Nardi, S
Allegranzi, B
Deganello, R
Vento, S
Lanzafame, M
Cazzadori, A
Bonora, S
Concia, E
机构
[1] UNIV VERONA, INST IMMUNOL & INFECT DIS, I-37100 VERONA, ITALY
[2] UNDP, WORLD BANK, WHO, SPECIAL PROGRAMME RES & TRAINING TROP DIS, GENEVA, SWITZERLAND
[3] KIREMBA HOSP, NGOZI, BURUNDI
关键词
malaria; Plasmodium falciparum; diagnosis; dipstick antigen capture assay; ParaSight(TM)-F test;
D O I
10.1016/S0035-9203(97)90257-1
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Three methods for the detection of Plasmodium falciparum infection in peripheral blood were compared during antimalarial treatment and follow-up in 32 Burundian patients: dipstick antigen capture assay, standard (TBF) and prolonged thick blood film examination (PTBF) (3x5 min and 3x20 min examination respectively). Parasitaemia was determined daily by comparison with total white blood cell counts (determined by Coulter counter) until no parasite was detected on 2 consecutive days by PTBF Cumulatively, 231 observations were made with each assay: 64 were negative and 167 positive by PTBF (59 had parasite counts less than or equal to 100/mu L). Compared to PTBF, the sensitivities of TBF and the dipstick assay were 1.0 for parasite counts >100/mu L and 0.458 and 0.966 respectively for counts less than or equal to 100/mu L. Overall, the dipstick assay was significantly more sensitive (0.988 vs. 0.808; P<0.001) but less specific (P=0.013) than TBF. The dipstick assay is of potential use for monitoring response to drug treatment and for detecting low parasitaemias.
引用
收藏
页码:403 / 405
页数:3
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