Functional proteomics revealed IL-1β amplifies TNF downstream protein signals in human synoviocytes in a TNF-independent manner

被引：55

作者：

Shen, Shan ^{[1
,2
]}

Guo, Jiahui ^{[2
]}

Luo, Yanzhang ^{[2
]}

Zhang, Wanling ^{[2
]}

Cui, Yizhi ^{[2
]}

Wang, Qing ^{[2
]}

Zhang, Zhiguang ^{[1
]}

Wang, Tong ^{[2
]}

机构：

[1] Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Dept Oral & Maxillofacial Surg, Guanghua Sch Stomatol,Hosp Stomatol, Guangzhou 510055, Guangdong, Peoples R China

[2] Jinan Univ, Guangdong Higher Educ Inst, Inst Life & Hlth Engn,Affiliated Hosp 1, Key Lab Funct Prot Res,Coll Life Sci & Technol, Guangzhou 510632, Guangdong, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 450卷 / 01期

关键词：

Synoviocytes; IL-1; beta; Proteomics; TNF-independent; p62; EARLY RHEUMATOID-ARTHRITIS; NF-KAPPA-B; DISEASE-ACTIVITY; OSTEOARTHRITIS; THERAPY; PATHOGENESIS; PATHWAYS; EFFICACY; ALPHA; MRI;

D O I：

10.1016/j.bbrc.2014.06.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

IL-1 beta is readily detectable in numerous joint inflammations. It can change the transcriptomic signature of fibroblast-like synoviocytes (FLS) of arthritis toward promoting migration and invasion that are relevant to arthritis progression. We hypothesize that IL-1 beta partially contributes to the onset of osteoarthritis (OA). We compared the tissue samples from OA and fracture subjects and found that IL-1 beta expression was significantly higher in the OA synovium, while TNF-alpha expression showed no significance. We demonstrated that IL-1 beta significantly increases the IL-6 and IL-8 secretions of human normal FLS; however, IL-1 beta does not induce TNF secretion. With metabolic labeling based proteomics and pathway analysis, we found that IL-1 beta significantly increases the TNF downstream protein expression in FLS even with complete absence of TNF and/or blocking of the NF-kappa B pathway. Among these proteins, we verified that p62 can differentiate the OA from fracture synovitis. In conclusion, we demonstrated that IL-1 beta can amplify the TNF downstream protein signals in human synoviocytes in a TNF-independent manner; in addition, p62 is a potential FLS biomarker for synovitis. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：538 / 544

页数：7

共 33 条

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Patients with rheumatoid arthritis treated with tumour necrosis factor antagonists increase their participation in the workforce: potential for significant long-term indirect cost gains (data from a population-based registry) [J].