Use of linear and multiple antigenic peptides in the immunodiagnosis of acute hepatitis A virus infection

被引：35

作者：

Gómara, MJ

Riedemann, S

Vega, I

Ibarra, H

Ercilla, G

Haro, I

机构：

[1] CSIC, IIQAB, Dept Quim Peptids & Prot, ES-08034 Barcelona, Spain

[2] Univ Austral Chile, Inst Med & Microbiol, Valdivia, Chile

[3] Hosp Clin Barcelona, IDIBAPS, Serv Immunol, Barcelona, Spain

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2000年 / 234卷 / 1-2期

关键词：

hepatitis A; immunodiagnosis; ELISA; synthetic peptides; multiple antigenic peptides; lipopeptides;

D O I：

10.1016/S0022-1759(99)00196-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The reactivities of two panels of anti-HAV human sera from geographically distinct areas (Chile and Spain) to synthetic peptides from the VP1, VP2 and VP3 hepatitis A virus capsid proteins were examined by an enzyme-linked immunosorbent assay (ELISA) procedure. Two and four branched multiple antigenic peptides (MAPs) and palmitoylated peptides were compared with free synthetic sequences for the detection of IgM anti-HAV antibodies in the two panels of human sera. Our results showed that acute hepatitis A patient sera recognized preferentially homogeneous two branched MAPs and palmitic acid conjugated peptides. The palmitoyl-derived VP3(110-121) peptide and the corresponding dimeric MAP were the most sensitive and appropriate for serological studies of HAV-infected patients by ELISA, sensitivity and specificity being higher than 90% and 95%, respectively. These peptide-based tests open up new avenues in the development of peptide-based immunosorbent assays for the detection of acute HAV disease. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：23 / 34

页数：12

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