Profiling early osmostress-dependent gene expression in Escherichia coli using DNA microarrays

被引:102
作者
Weber, A [1 ]
Jung, K [1 ]
机构
[1] Univ Osnabruck, Fachbereich Biol Chem, Abt Mikrobiol, D-49069 Osnabruck, Germany
关键词
D O I
10.1128/JB.184.19.5502-5507.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
DNA macroarray technology was used to monitor early transcriptional alterations of Escherichia coli in response to an osmotic upshift imposed by the addition of 0.4 M NaCl. Altered mRNA levels of 152 genes were detected; 45 genes showed increased expression while the expression of the remaining 107 genes was reduced. Northern blot analysis of several selected genes differing in their relative expression values confirmed the results obtained by the array technology.
引用
收藏
页码:5502 / 5507
页数:6
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