Functional analysis of DNA gyrase mutant enzymes carrying mutations at position 88 in the A subunit found in clinical strains of Mycobacterium tuberculosis resistant to fluoroquinolones

被引：40

作者：

Matrat, Stephanie

Veziris, Nicolas

Mayer, Claudine

Jarlier, Vincent

Truffot-Pernot, Chantal

Camuset, Juliette

Bouvet, Elisabeth

Cambau, Emmanuelle

Aubry, Alexandra

机构：

[1] Univ Paris 06, Assistance Publ Hop Paris, Bacteriol Lab, Ctr Reference Mycobacteries & Resistance Mycobact, Paris, France

[2] INSERM, U655, LRMA, Paris, France

[3] Ctr Hosp Argenteuil, Serv Pneumol, Argenteuil, France

[4] Assistance Publ Hop Paris, Fac Med Rene Diderot, Serv Malad Infect, Paris, France

[5] Univ Paris 12, CHU Henri Mondor, Lab Bacteriol Virol Hyg, Assistance Publ Hop Paris, Creteil, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2006年 / 50卷 / 12期

关键词：

D O I：

10.1128/AAC.00944-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We investigated the enzymatic efficiency and inhibition by quinolones of Mycobacterium tuberculosis DNA gyrases carrying the previously described GyrA G88C mutation and the novel GyrA G88A mutation harbored by two multidrug-resistant clinical strains and reproduced by site-directed mutagenesis. Fluoroquinolone MICs and 50% inhibitory concentrations for both mutants were 2- to 43-fold higher than for the wild type, demonstrating that these mutations confer fluoroquinolone resistance in M. tuberculosis.

引用

页码：4170 / 4173

页数：4

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