Controlled Manipulation of Bacteriophages Using Single-Virus Force Spectroscopy

被引：19

作者：

Alsteens, David ^{[2
]}

Pesavento, Emanuele ^{[1
]}

Cheuvart, Gilles ^{[2
]}

Dupres, Vincent ^{[2
]}

Trabelsi, Heykel ^{[1
]}

Soumillion, Patrice ^{[1
]}

Dufrene, Yves F. ^{[2
]}

机构：

[1] Univ Catholique Louvain, Inst Sci Vie, Lab Ingn Prot & Peptides, B-1348 Louvain, Belgium

[2] Univ Catholique Louvain, Unite Chim Interfaces, B-1348 Louvain, Belgium

来源：

ACS NANO | 2009年 / 3卷 / 10期

关键词：

phage display; bacteriophages; single virus manipulation; atomic force microscopy; force spectroscopy; ANTIGEN-BINDING FORCES; ENERGY LANDSCAPES; HUMAN RHINOVIRUS; LIVE CELLS; MICROSCOPY; PHAGE; RESOLUTION; NANOWIRES; MOLECULES; COMPLEX;

D O I：

10.1021/nn900778t

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

A method is described for the site-directed manipulation of single filamentous bacteriophages, by using phage display technology and atomic force microscopy. f1 filamentous bacteriophages were genetically engineered to display His-tags on their pIX tail. Following adsorption on nitrilotriacetate-terminated surfaces, force spectroscopy with tips bearing monoclonal anti-PIII antibodies was used to pull on individual phages via their PIII head. Analysis of the force-extension profiles revealed that upon pulling, the phages are progressively straightened into an extended orientation until rupture of the anti-PIII/PIII complex. The single-virus manipulation technique presented here provides new opportunities for understanding the forces driving cell-virus and material-virus interactions, and for characterizing the binding properties of polypeptide sequences or proteins selected by the phage display technology.

引用

页码：3063 / 3068

页数：6

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