Mammalian glial cells in culture synthesize acetylcholine

In the present study we demonstrate that acetylcholine is synthesized by cultured mammalian glial cells identified by cell-type specific markers. Primary cultures of rat brain astrocytes or microglia contained 2.0 and 1.6 pmol acetylcholine/10(6) cells on average respectively. Astrocyte cultures established from neonatal mouse brain contained even more acetylcholine (about 80 pmol acetylcholine/10(6) cells). Primary;cultures of rat brain astrocytes showed choline acetyltransferase (ChAT) enzyme activity of 3 nmol/mg protein/h; ChAT activity was blocked by 10 mu M bromoacetylcholine. In conclusion, these data demonstrate the synthesis of the ''neurotransmitter'' acetylcholine in cultured glial cells, a finding which opens a new view upon the role of acetylcholine in mammalian brain.