Rapid identification of nontypeable and serotype b Haemophilus influenzae from nasopharyngeal secretions by the multiplex PCR

被引:10
作者
Billal, Dewan S.
Hotomi, Muneki
Suzumoto, Masaki
Yamauchi, Kazuma
Kobayashi, Intetsu
Fujihara, Keiji
Yamanaka, Noboru
机构
[1] Wakayama Med Univ, Dept Otolaryngol Head & Neck Surg, Wakayama 6410012, Japan
[2] Mitsubishi Kagaku Bioclin Labs, Tokyo, Japan
关键词
Haemophilus influenzae; nontypeable; serotype; multiplex PCR; slide agglutination test;
D O I
10.1016/j.ijporl.2006.10.009
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
Objective: Heamophilus influenzae (H. influenzae) is an important pathogen responsible for both invasive and non-invasive infectious diseases. While encapsulated type b strain recognized as a major cause of severe invasive diseases, nontypeable strains are the major causes of non-invasive infectious diseases. Detection of this pathogen from nasopharyngeal secretions (NIPS) is important. Methods: We developed a multiplex polymerase chain reaction (PCR) for rapid identification of nontypeable and serotype b H. iniluenzae from nasopharyngeal secretions. Results: A total 25 nasopharyngeal secretions were evaluated in this study. The multiplex PCR provided rapid and unequivocal results for determining either nontypeable or encapsulated typeable especially type b strains including a determination of beta-lactamase productions. Conclusion: The multiplex PCR based serotyping provided more reliable results than slide agglutination test (SAT) and is a valuable and expeditious method for identification of H. influenzae with determining capsular serotypes. (C) 2006 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:269 / 274
页数:6
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