Relaxin-like factor (RLF) mRNA expression in the fallow deer

被引:24
作者
Hombach-Klonisch, S
Kauffold, J
Rautenberg, T
Steger, K
Tetens, F
Fischer, B
Klonisch, T
机构
[1] Univ Halle Wittenberg, Fac Med, Dept Anat & Cell Biol, D-06097 Halle, Germany
[2] Univ Leipzig, Fac Vet Med, Large Anim Clin Theriogenol & Ambulatory Serv, D-04103 Leipzig, Germany
[3] Univ Giessen, Inst Vet Anat, D-35392 Giessen, Germany
关键词
relaxin-like factor; RLF; Ins13; deer; ovary; placenta; testis; MHC class I;
D O I
10.1016/S0303-7207(99)00190-2
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Employing RT- and RACE-PCR on RNA isolated from testicular tissue, we have cloned the coding cDNA sequence for the RLF, also known as Ins13, of the fallow deer. The RLF coding sequence consisted of 396 bp encoding a peptide of 131 amino acids and shared highest homology with bovine, sheep and goat RLF. Northern analysis revealed a single 0.9 kb transcript in the deer testis. There is only one RLF gene in the deer genome. Nonradioactive in situ hybridization revealed the Leydig cells to be the sole source for RLF mRNA in the deer testis. In the non-pregnant uterus, RLF transcripts were located in the luminal and glandular epithelium of the endometrium. Within the ovary of the pregnant doe, follicular theca interns cells and the corpus luteum expressed RLF transcripts. In uteroplacental tissues, luminal and glandular epithelium, fetal uninucleate and binucleate trophoblast cells (BNC) of the basic villous trophoblast layer expressed RLF mRNA. BNC located at the apical trophoblast layer or the tip of the fetal villus were devoid of RLF transcripts. Pseudostratified trophoblast cells at the base of fetal villi coexpressed RLF mRNA and immunoreactive MHC class Ib molecules. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:147 / 158
页数:12
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