Detection of Plum pox potyvirus in illegally imported plums intercepted at Sydney International Airport

被引：6

作者：

Davis, K

Rodoni, B

Knox, C

Sarec, R

Moran, J

机构：

[1] AQIS, Plant Quarantine Stn, Eastern Creek, NSW 2766, Australia

[2] Agr Victoria, Inst Hort Dev, Knoxfield, Vic 3180, Australia

来源：

AUSTRALASIAN PLANT PATHOLOGY | 2002年 / 31卷 / 03期

关键词：

D O I：

10.1071/AP02035

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plum pox potyvirus (PPV) was detected in an illegal consignment of plum budwood and fruit intercepted by Australian Quarantine and Inspection Service (AQIS) inspectors at Sydney International Airport. PPV was detected in leaf, bark and peduncle tissue using enzyme linked immunosorbent assay (ELISA), reverse transcription-polymerase chain reaction (RT-PCR) and electron microscopy. The strain of PPV detected was the D strain as indicated by ELISA and restriction enzyme analysis of the P1-P2 243 bp PCR product.

引用

页码：313 / 314

页数：2

共 5 条

[1]

Levy L., 2000, Plum pox potyvirus disease of stone fruits

[2] Improved RNA extraction from woody plants for the detection of viral pathogens by reverse transcription-polymerase chain reaction [J].