A standardized plaque reduction assay for determination of drug susceptibilities of cytomegalovirus clinical isolates

被引：105

作者：

Landry, ML

Stanat, S

Biron, K

Brambilla, D

Britt, W

Jokela, J

Chou, SW

Drew, WL

Erice, A

Gilliam, B

Lurain, N

Manischewitz, J

Miner, R

Nokta, M

Reichelderfer, P

Spector, S

Weinberg, A

Yen-Lieberman, B

Crumpacker, C

机构：

[1] Yale Univ, Sch Med, Dept Lab Med, New Haven, CT 06520 USA

[2] Glaxo Wellcome, Res Triangle Pk, NC USA

[3] New England Res Inst, Watertown, MA 02172 USA

[4] Univ Alabama, Birmingham, AL USA

[5] Univ Oregon, Portland, OR USA

[6] Univ Calif San Francisco, San Francisco, CA 94143 USA

[7] Univ Minnesota, Minneapolis, MN USA

[8] Univ N Carolina, Chapel Hill, NC USA

[9] Rush Med Coll, Chicago, IL 60612 USA

[10] US FDA, Bethesda, MD 20014 USA

[11] NIAID, Div Aids, NIH, Bethesda, MD 20892 USA

[12] Univ Texas, Galveston, TX 77555 USA

[13] Univ Calif San Diego, San Diego, CA 92103 USA

[14] Cleveland Clin Fdn, Cleveland, OH 44195 USA

[15] Harvard Univ, Boston, MA 02115 USA

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2000年 / 44卷 / 03期

关键词：

D O I：

10.1128/AAC.44.3.688-692.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Twelve laboratories collaborated in formulating and testing a standardized plaque reduction assay for cyto megalovirus (CMV) cell-associated clinical isolates, Four characterized and plaque-purified CMV strains, as well as six coded clinical isolates obtained after antiviral therapy, were distributed and tested. Good agreement was obtained for four of the clinical isolates, but a broad distribution of results was obtained for two Isolates. Analysis of these results indicates the problems associated,vith clinical isolates, including the large genetic variability and the highly cell-associated phenotype. This collaborative effort, by addressing these problems, represents a significant step toward the development of a standardized assay.

引用

页码：688 / 692

页数：5

共 13 条

[1] ANALYSIS OF THE UL97 PHOSPHOTRANSFERASE CODING SEQUENCE IN CLINICAL CYTOMEGALOVIRUS ISOLATES AND IDENTIFICATION OF MUTATIONS CONFERRING GANCICLOVIR RESISTANCE [J].