DNA damage induced by hypochlorite and hypobromite with reference to inflammation-associated carcinogenesis

被引：44

作者：

Ohnishi, S ^{[1
]}

Murata, M ^{[1
]}

Kawanishi, S ^{[1
]}

机构：

[1] Mie Univ, Sch Med, Dept Hyg, Tsu, Mie 5148507, Japan

来源：

CANCER LETTERS | 2002年 / 178卷 / 01期

关键词：

DNA damage; hypochlorite; hypobromite; glutathione;

D O I：

10.1016/S0304-3835(01)00812-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Hypohalites (OCl-, OBr-) are formed at inflammation sites as antimicrobial agents. OCl- is also used for the disinfection of water supplies and the association of drinking chlorinated water with cancer risk is pointed out. In this study, OCl- itself induced 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) formation, while OBr- damaged DNA only when glutathione (GSH) was added. OCl- caused oxidative DNA damage more efficiently than OBr-/GSH. In experiment with P-32-labeled DNA fragments, OCl- strongly caused piperidine-labile sites at guanine residues than piperidine-inert 8-oxodG, whereas OBr-/ GSH caused no piperidine-labile sites. Endogenous OCl- may play a role in genotoxicity close to the site of inflammation. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

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页码：37 / 42

页数：6

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