Experimental obstructive jaundice alters claudin-4 expression in intestinal mucosa: Effect of bombesin and neurotensin

被引:9
作者
Assimakopoulos, Stelios F.
Vagianos, Constantine E.
Charonis, Aristides S.
Alexandris, Ilias H.
Spiliopoulou, Iris
Thomopoulos, Konstantinos C.
Nikolopoulou, Vassiliki N.
Scopa, Chrisoula D.
机构
[1] Univ Patras, Sch Med, Dept Internal Med, Div Gastroenterol, GR-26110 Patras, Greece
[2] Univ Patras, Sch Med, Dept Surg, GR-26110 Patras, Greece
[3] Acad Athens, Fdn Biomed Res, Ctr Basic Res, Sect Histol, Athens, Greece
[4] Univ Patras, Sch Med, Dept Microbiol, GR-26110 Patras, Greece
[5] Univ Patras, Sch Med, Dept Pathol, GR-26110 Patras, Greece
关键词
obstructive jaundice; tight junctions; claudin-4; intestinal permeability; intestinal barrier; regulatory pepticles; bombesin; neurotensin;
D O I
10.3748/wjg.v12.i21.3410
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
AIM: To investigate the influence of experimental obstructive jaundice and exogenous bombesin (BBS) and neurotensin (NT) administration on the expression of the tight junction (TJ)-protein claudin-4 in intestinal epithelium of rats. METHODS: Forty male Wistar rats were randomly divided into five groups: I = controls, II = sham operated, III = bile duct ligation (BDL), IV = BDL+BBS (30 mu g/kg per d), V = BDL+NT (300 mu g/kg per d). At the end of the experiment on d 10, endotoxin was measured in portal and aortic blood. Tissue sections of the terminal ileum were examined histologically and immunohistochemically for evaluation of claudin-4 expression in intestinal epithelium. RESULTS: Obstructive jaundice led to intestinal barrier failure demonstrated by significant portal and aortic endotoxemia. Claudin-4 expression was significantly increased in the upper third, of the villi in jaundiced rats and an upregulation of its lateral distribution was noted. Administration of BBS or NT restored claudin-4 expression to the control state and significantly reduced portal and aortic endotoxemia. CONCLUSION: Experimental obstructive jaundice increases claudin-4 expression in intestinal epithelium, which may be a key factor contributing to the disruption of the mucosal barrier. Gut regulatory peptides BBS and NT can prevent this alteration and reduce portal and systemic endotoxemia. (C) 2006 The WJG Press. All rights reserved.
引用
收藏
页码:3410 / 3415
页数:6
相关论文
共 32 条
[1]   Bombesin improves burn-induced intestinal injury in the rat [J].
Alican, I ;
Ünlüer, EE ;
Yegen, C ;
Yegen, BÇ .
PEPTIDES, 2000, 21 (08) :1265-1269
[2]   TIGHT JUNCTIONS AND THE MOLECULAR-BASIS FOR REGULATION OF PARACELLULAR PERMEABILITY [J].
ANDERSON, JM ;
VANITALLIE, CM .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 1995, 269 (04) :G467-G475
[3]   Bombesin and neurotensin reduce endotoxemia, intestinal oxidative stress, and apoptosis in experimental obstructive jaundice [J].
Assimakopoulos, SF ;
Scopa, CD ;
Zervoudakis, G ;
Mylonas, PG ;
Georgiou, C ;
Nikolopoulou, V ;
Vagianos, CE .
ANNALS OF SURGERY, 2005, 241 (01) :159-167
[4]   Experimental obstructive jaundice disrupts intestinal mucosal barrier by altering occludin expression: Beneficial effect of bombesin and neurotensin [J].
Assimakopoulos, SF ;
Scopa, CD ;
Charonis, A ;
Spiliopoulou, I ;
Georgiou, C ;
Nikolopoulou, V ;
Vagianos, CE .
JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS, 2004, 198 (05) :748-757
[5]   θ isoform of protein kinase C alters barrier function in intestinal epithelium through modulation of distinct claudin isotypes:: A novel mechanism for regulation of permeability [J].
Banan, A ;
Zhang, LJ ;
Shaikh, M ;
Fields, JZ ;
Choudhary, S ;
Forsyth, CB ;
Farhadi, A ;
Keshavarzian, A .
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2005, 313 (03) :962-982
[6]   Chronic inflammatory pain leads to increased blood-brain barrier permeability and tight junction protein alterations [J].
Brooks, TA ;
Hawkins, BT ;
Huber, JD ;
Egleton, RD ;
Davis, TP .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2005, 289 (02) :H738-H743
[7]   ROLE OF BOMBESIN ON GUT MUCOSAL GROWTH [J].
CHU, KU ;
EVERS, BM ;
ISHIZUKA, J ;
TOWNSEND, CM ;
THOMPSON, JC .
ANNALS OF SURGERY, 1995, 222 (01) :94-100
[8]   EFFECT OF NEUROTENSIN ON GUT MUCOSAL GROWTH IN RATS WITH JEJUNAL AND ILEAL THIRY-VELLA FISTULAS [J].
CHUNG, DH ;
EVERS, BM ;
SHIMODA, I ;
TOWNSEND, CM ;
RAJARAMAN, S ;
THOMPSON, JC .
GASTROENTEROLOGY, 1992, 103 (04) :1254-1259
[9]   Role of the gut in the pathophysiology of extrahepatic biliary obstruction [J].
Clements, WDB ;
Parks, R ;
Erwin, P ;
Halliday, MI ;
Barr, J ;
Rowlands, BJ .
GUT, 1996, 39 (04) :587-593
[10]  
DEITCH EA, 1990, ARCH SURG-CHICAGO, V125, P403