Leishmania (V) guyanensis: Isolation and characterization of glucantime-resistant cell lines

被引：23

作者：

FerreiraPinto, KC ^{[1
]}

MirandaVilela, AL ^{[1
]}

Anacleto, C ^{[1
]}

Fernandes, APSM ^{[1
]}

Abdo, MCB ^{[1
]}

PetrilloPeixoto, ML ^{[1
]}

Moreira, ESA ^{[1
]}

机构：

[1] UNIV FED MINAS GERAIS,INST CIENCIAS BIOL,DEPT MICROBIOL,BR-30161910 BELO HORIZONT,MG,BRAZIL

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1996年 / 42卷 / 09期

关键词：

Leishmania; glucantime resistance; pentavalent antimony; gene amplification;

D O I：

10.1139/m96-121

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A glucantime sensitive Leishmania (V.) guyanensis strain was used to obtain in vitro resistant cell lines, by increments in glucantime concentrations employing both one step and stepwise protocols. Whereas the effective concentration of drug that inhibited the growth of wild type cells by 50% (EC(50) value) was 0.20 mg Sb-v/mL, the resistant cells were able to grow in glucantime concentrations greater than 8.0 mg/mL. The resistant cell lines were partially characterized by their in vitro response to glucantime, the stability of resistance phenotype, cross resistance to a range of drugs, and also by the analysis of total DNA fragments generated by restriction endonucleases and blot hybridization. Amplified DNA sequence similar to a P-glycoprotein analog from Leishmania tarentolae (ltpgpA gene) was observed in all the resistant cell lines obtained through the one-step protocol. These cell lines showed cross resistance to heavy metals but were sensitive to puromycin, vinblastine, and pentostam.

引用

页码：944 / 949

页数：6

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