The use of carboxyfluorescein diacetate succinimidyl ester to determine the site, duration and cell type responsible for antigen presentation in vivo

被引：28

作者：

Mintern, J

Li, M

Davey, GM

Blanas, E

Kurts, C

Carbone, FR

Heath, WR

机构：

[1] Walter & Eliza Hall Inst Med Res, Div Immunol, Melbourne, Vic 3050, Australia

[2] Queensland Inst Med Res, Cooperat Res Ctr Vaccine Technol, Herston, Qld 4006, Australia

[3] Monash Univ Sch Med, Dept Pathol & Immunol, Prahran, Vic, Australia

来源：

IMMUNOLOGY AND CELL BIOLOGY | 1999年 / 77卷 / 06期

关键词：

antigen presentation; CD8-positive T lymphocytes; ovalbumin; transgenic mice;

D O I：

10.1046/j.1440-1711.1999.00868.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This report examines the use of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) to determine the site, duration and cell type responsible for antigen presentation in vivo. Evidence that CFSE-labelled T cells can be used to determine where various types of antigens are presented, including auto-antigens, oral antigens and cell-associated foreign antigens, is provided. Using this technique, the length of time antigen is presented after acquisition by APC was measured. Finally, CFSE labelling was used to identify the origin of the APC responsible for different forms of antigen presentation.

引用

页码：539 / 543

页数：5

共 19 条

[1] Defective TCR expression in transgenic mice constructed using cDNA-based α- and β-chain genes under the control of heterologous regulatory elements [J].