Ku heterodimer binds to both ends of the Werner protein and functional interaction occurs at the Werner N-terminus

被引:87
作者
Karmakar, P
Snowden, CM
Ramsden, DA
Bohr, VA
机构
[1] NIA, Lab Mol Gerontol, IRP, NIH, Baltimore, MD 21224 USA
[2] Univ N Carolina, Lineberger Comprehens Canc Ctr, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA
[3] Univ N Carolina, Curriculum Genet & Mol Biol, Chapel Hill, NC 27599 USA
关键词
D O I
10.1093/nar/gkf482
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The human Werner syndrome protein, WRN, is a member of the RecQ helicase family and contains 3'-->5' helicase and 3'-->5' exonuclease activities. Recently, we showed that the exonuclease activity of WRN is greatly stimulated by the human Ku heterodimer protein. We have now mapped this interaction physically and functionally. The Ku70 subunit specifically interacts with the N-terminus (amino acids 1-368) of WRN, while the Ku80 subunit interacts with its C-terminus (amino acids 940- 1432). Binding between Ku70 and the N-terminus of WRN (amino acids 1-368) is sufficient for stimulation of WRN exonuclease activity. A mutant Ku heterodimer of full-length Ku80 and truncated Ku70 (amino acids 430-542) interacts with C-WRN but not with N-WRN and cannot stimulate WRN exonuclease activity. This emphasizes the functional significance of the interaction between the N-terminus of WRN and Ku70. The interaction between Ku80 and the C-terminus of WRN may modulate some other, as yet unknown, function. The strong interaction between Ku and WRN suggests that these two proteins function together in one or more pathways of DNA metabolism.
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页码:3583 / 3591
页数:9
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