Genetic diversity and phylogeny of Japanese sake-brewing rice as revealed by AFLP and nuclear and chloroplast SSR markers

被引:52
作者
Hashimoto, Z
Mori, N
Kawamura, M
Ishii, T
Yoshida, S
Ikegami, M
Takumi, S
Nakamura, C
机构
[1] Kobe Univ, Fac Agr, Dept Biol & Environm Sci, Lab Plant Genet,Nada Ku, Kobe, Hyogo 6578501, Japan
[2] Kobe Univ, Grad Sch Sci & Technol, Nada Ku, Kobe, Hyogo 6578501, Japan
[3] Kobe Univ, Fac Agr, Dept Plant Resources Sci, Lab Plant Breeding,Nada Ku, Kobe, Hyogo 6578501, Japan
[4] Hyogo Inst Agr Forestry & Fishery, Kasai, Hyogo 6790198, Japan
关键词
D O I
10.1007/s00122-004-1794-6
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Japanese rice (Oryza sativa L.) cultivars that are strictly used for the brewing of sake (Japanese rice wine) represent a unique and traditional group. These cultivars are characterized by common traits such as large grain size with low protein content and a large, central white-core structure. To understand the genetic diversity and phylogenetic characteristics of sake-brewing rice, we performed amplified fragment length polymorphism and simple sequence repeat analyses, using 95 cultivars of local and modern sake-brewing rice together with 76 cultivars of local and modern cooking rice. Our analysis of both nuclear and chloroplast genome polymorphisms showed that the genetic diversity in sake-brewing rice cultivars was much smaller than the diversity found in cooking rice cultivars. Interestingly, the genetic diversity within the modern sake-brewing cultivars was about twofold higher than the diversity within the local sake-brewing cultivars, which was in contrast to the cooking cultivars. This is most likely due to introgression of the modern cooking cultivars into the modern sake-brewing cultivars through breeding practices. Cluster analysis and chloroplast haplotype analysis suggested that the local sake-brewing cultivars originated monophyletically in the western regions of Japan. Analysis of variance tests showed that several markers were significantly associated with sake-brewing traits, particularly with the large white-core structure.
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页码:1586 / 1596
页数:11
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