Apoptosis in Hep2 cells treated with etoposide and colchicine

被引:14
作者
Cervinka, M
Cerman, J
Rudolf, E
机构
[1] Charles Univ Prague, Dept Med Biol & Genet, Fac Med Kradec Kralove, Prague 50038, Czech Republic
[2] Charles Univ Prague, Dept Med Biochem, Fac Med Hradec Kralove, Prague, Czech Republic
来源
CANCER DETECTION AND PREVENTION | 2004年 / 28卷 / 03期
关键词
time-lapse videomicroscopy; blebbing; caspase-3; tubulin; actin;
D O I
10.1016/j.cdp.2004.03.002
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
When malignant cells undergo apoptosis, they exhibit many distinct patterns of behavior, with blebbing being one of the most spectacular and mysterious features. Despite huge advancements in our understanding of cell death, the mechanisms of apoptosis associated blebbing have not been elucidated. In order to verify the putative involvement of actin and tubulin in this process, Hep2 cells were treated with a combination of etoposide (10 mug/ml) and colchicine (0.2 mug/ml) for 24 h. Blebbing was analyzed using immunofluorescence staining of actin and tubulin, and the course of apoptosis Was followed by time-lapse videomicroscopy, immunofluorescence detection of caspase-3 and cytokeratin fragment 18. The results indicate that microfilaments (actin) and not microtubules (tubulin) are involved in blebbing of Hep2 cells. Furthermore, despite the different mechanisms by which both chemicals act, their combined effects are not additive, but rather eliminate each other. (C) 2004 International Society for Preventive Oncology. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:214 / 226
页数:13
相关论文
共 39 条
[1]   Detection of apoptosis induced by topoisomerase inhibitors and serum deprivation in Syrian hamster embryo cells [J].
Alexandre, S ;
Rast, C ;
Nguyen-Ba, G ;
Vasseur, P .
EXPERIMENTAL CELL RESEARCH, 2000, 255 (01) :30-39
[2]   Etoposide-induced activation of c-jun N-terminal kinase (JNK) correlates with drug-induced apoptosis in salivary gland acinar cells [J].
Anderson, SM ;
Reyland, ME ;
Hunter, S ;
Deisher, LM ;
Barzen, KA ;
Quissell, DO .
CELL DEATH AND DIFFERENTIATION, 1999, 6 (05) :454-462
[3]   Genotoxicity of several clinically used topoisomerase II inhibitors [J].
Boos, G ;
Stopper, H .
TOXICOLOGY LETTERS, 2000, 116 (1-2) :7-16
[4]  
CARNEY DN, 1991, CANCER, V67, P299, DOI 10.1002/1097-0142(19910101)67:1+<299::AID-CNCR2820671315>3.0.CO
[5]  
2-K
[6]  
CERVINKA M, 1999, ROLE MITOCHONDRIA AP, P33
[7]  
COHEN GM, 1992, BIOCHEM J, P331
[8]   Tumor necrosis factor-induced apoptosis stimulates p53 accumulation and p21WAF1 proteolysis in ME-180 cells [J].
Donato, NJ ;
Perez, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (09) :5067-5072
[9]  
Gallaher B, 2000, CELL MOL BIOL, V46, P79
[10]   Cytochrome c release and caspase-3 activation during colchicine-induced apoptosis of cerebellar granule cells [J].
Gorman, AM ;
Bonfoco, E ;
Zhivotovsky, B ;
Orrenius, S ;
Ceccatelli, S .
EUROPEAN JOURNAL OF NEUROSCIENCE, 1999, 11 (03) :1067-1072