The fluorescent cholesterol analog dehydroergosterol induces liquid-ordered domains in model membranes

被引：31

作者：

Garvik, Olav ^{[2
]}

Benediktson, Peter ^{[2
]}

Simonsen, Adam Cohen ^{[2
]}

Ipsen, John Hjort ^{[2
]}

Wustner, Daniel ^{[1
]}

机构：

[1] Univ So Denmark, Dept Biochem & Mol Biol, DK-5230 Odense M, Denmark

[2] Univ So Denmark, Dept Chem & Phys, MEMPHYS Ctr Biomembrane Phys, DK-5230 Odense M, Denmark

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 2009年 / 159卷 / 02期

关键词：

Sterol; Membrane; Fluorescence; Bilayer; Microscopy; Intracellular transport; STEROL STRUCTURE DETERMINES; DYNAMICS; BEHAVIOR; BILAYERS; PROBES; PHASES;

D O I：

10.1016/j.chemphyslip.2009.03.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The fluorescent sterol dehydroergosterol (DHE) is often used as a marker for cholesterol in cellular studies. We show by vesicle fluctuation analysis that DHE has a lower ability than cholesterol to stiffen lipid bilayers suggesting less efficient packing with phospholipid acyl chains. Despite this difference, we found by fluorescence and atomic force microscopy, that DHE induces liquid-ordered/-disordered coexistent domains in giant unilamellar vesicles (GUVs) and supported bilayers made of dipalmitoylphosphatidylcholine (DPPC). dioleylphosphatidylcholine (DOPC) and DHE or cholesterol. DHE-induced phases have a height difference of 0.9-1 nm similar as known for cholesterol-containing domains. DHE not only promotes formation of liquid-liquid immiscibility but also shows strong partition preference for the liquid-ordered phase further supporting its suitability as cholesterol probe. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：114 / 118

页数：5

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