In vitro remodeling of tumor vascular endothelial cells using conditioned medium from various tumor cells and their sensitivity to TNF-α

被引:16
作者
Kamada, H [1 ]
Tsutsumi, Y [1 ]
Kihira, T [1 ]
Tsunoda, S [1 ]
Yamamoto, Y [1 ]
Mayumi, T [1 ]
机构
[1] Osaka Univ, Grad Sch Pharmaceut Sci, Dept Biopharmaceut, Suita, Osaka 5650871, Japan
基金
日本学术振兴会;
关键词
tumor necrosis factor-alpha; tumor endothelial cell; conditioned medium; antitumor effect; antitumor mechanism;
D O I
10.1006/bbrc.2000.2202
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Prevention of tumor-associated blood vessel formation (angiogenesis) is potentially powerful strategy to treat cancer. We found that tumor vascular endothelial cells were rearranged in vitro with conditioned culture medium derived from tumor cells and compared the sensitivity to the effects of TNF-alpha between normal and tumor endothelial cells, incubation with tumor (Meth-A, Colon26)-derived conditioned medium showed that no effect was observed on cell growth. Tumor cells (Meth-A, Colon26, and B16BL6) only showed no sensitivity to TNF-alpha. Normal and control endothelial cells in culture showed little cytotoxicity in response to TNF-alpha treatment, but marked cytotoxicity of TNF-alpha was observed in endothelial cells cultured with tumor-derived conditioned medium. Sensitivity to TNF-alpha was different depending on the type of tumor from. which the conditioned medium was derived. This difference in sensitivity was assumed to be due to the in vivo sensitivity to TNF-alpha. The results of this study suggested that the sensitivity of tumors to TNF-alpha is controlled by the sensitivity of tumor vasculature. (C) 2000 Academic Press.
引用
收藏
页码:809 / 813
页数:5
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