Thyroid status co-regulates thyroid hormone receptor and co-modulator genes specifically in the hypothalamus

被引：19

作者：

Clerget-Froidevaux, MS ^{[1
]}

Seugnet, I ^{[1
]}

Demeneix, BA ^{[1
]}

机构：

[1] Museum Natl Hist Nat, CNRS, UMR 5166, USM 501,Dept Regulat Dev & Divers Mol, F-75231 Paris 5, France

来源：

FEBS LETTERS | 2004年 / 569卷 / 1-3期

关键词：

thyroid hormones; TRH transcription; gene synexpression; semi-quantitative multiplex PCR; tissue specific gene regulation; paraventricular nucleus of the hypothalamus;

D O I：

10.1016/j.febslet.2004.05.076

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Regulation of Thyrotropin Releasing Hormone (TRH) transcription in the hypothalamus represents the central control point of thyroid function. To examine the expression of potential TRH regulatory components, we simultaneously amplified, by semi-quantitative multiplex PCR system, nine key genes from less than or equal to100 ng total RNA from two brain areas (hypothalamus and cortex) under different thyroid states. Expression of TR1 and TR2 isoforms, key elements in TRH regulation, was modified by thyroid status in the hypothalamus but not in the cortex. Similarly, hypothyroidism increased specifically hypothalamic levels of three co-modulator genes. This study provides the first demonstration of tissue specific co-regulation of a set of genes by thyroid status within a defined brain area. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：341 / 345

页数：5

共 21 条

[1] Critical role for thyroid hormone receptor β2 in the regulation of paraventricular thyrotropin-releasing hormone neurons [J].