Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery

被引：147

作者：

Fischer, T

Rodríguez-Navarro, S

Pereira, G

Rácz, A

Schiebel, E

Hurt, E

机构：

[1] Univ Heidelberg BZH, Zentrum Biochem, D-69120 Heidelberg, Germany

[2] Christie Hosp NHS Trust, Paterson Inst Canc Res, Manchester M20 4BX, Lancs, England

来源：

NATURE CELL BIOLOGY | 2004年 / 6卷 / 09期

关键词：

D O I：

10.1038/ncb1163

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Centrins are calmodulin-like proteins that function in the duplication of microtubule-organizing centres. Here we describe a new function of the yeast centrin Cdc31. We show that overproduction of a sequence, termed CID, in the carboxy-terminal domain of the nuclear export factor Sac3 titrates Cdc31, causing a dominant-lethal phenotype and a block in spindle pole body (SPB) duplication. Under normal conditions, the CID motif recruits Cdc31 and Sus1 (a subunit of the SAGA transcription complex) to the Sac3-Thp1 complex, which functions in mRNA export together with specific nucleoporins at the nuclear basket. A previously reported cdc31 temperature-sensitive allele, which is neither defective in SPB duplication nor Kic1 kinase activation, induces mRNA export defects. Thus, Cdc31 has an unexpected link to the mRNA export machinery.

引用

页码：840 / U4

页数：10

共 42 条

[1] Centrosome protein centrin 2/caltractin 1 is part of the xeroderma pigmentosum group C complex that initiates global genome nucleotide excision repair
Araki, M
Masutani, C
Takemura, M
Uchida, A
Sugasawa, K
Kondoh, J
Ohkuma, Y
Hanaoka, F
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (22) : 18665 - 18672
[2] Identification of a 60S preribosomal particle that is closely linked to nuclear export
Bassler, J
Grandi, P
Gadal, O
Lessmann, T
Petfalski, E
Tollervey, D
Lechner, J
Hurt, E
[J]. MOLECULAR CELL, 2001, 8 (03) : 517 - 529
[3] YEAST GENE REQUIRED FOR SPINDLE POLE BODY DUPLICATION - HOMOLOGY OF ITS PRODUCT WITH CA-2+-BINDING PROTEINS
BAUM, P
FURLONG, C
BYERS, B
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (15) : 5512 - 5516
[4] An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast
Bellí, G
Garí, E
Piedrafita, L
Aldea, N
Herrero, E
[J]. NUCLEIC ACIDS RESEARCH, 1998, 26 (04) : 942 - 947
[5] DIRECT INTERACTION BETWEEN YEAST SPINDLE POLE BODY COMPONENTS - KAR1P IS REQUIRED FOR CDC31P LOCALIZATION TO THE SPINDLE POLE BODY
BIGGINS, S
ROSE, MD
[J]. JOURNAL OF CELL BIOLOGY, 1994, 125 (04) : 843 - 852
[6] A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae
Chávez, S
Beilharz, T
Rondón, AG
Erdjument-Bromage, H
Tempst, P
Svejstrup, JQ
Lithgow, T
Aguilera, A
[J]. EMBO JOURNAL, 2000, 19 (21) : 5824 - 5834
[7] Spc42p: A phosphorylated component of the S-cerevisiae spindle pole body (SPB) with an essential function during SPB duplication
Donaldson, AD
Kilmartin, JV
[J]. JOURNAL OF CELL BIOLOGY, 1996, 132 (05) : 887 - 901
[8] Regulation of calcium signals in the nucleus by a nucleoplasmic reticulum
Echevarria, W
Leite, MF
Guerra, MT
Zipfel, WR
Nathanson, MH
[J]. NATURE CELL BIOLOGY, 2003, 5 (05) : 440 - 446
[9] The nuclear pore complex: Nucleocytoplasmic transport and beyond
Fahrenkrog, B
Aebi, U
[J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2003, 4 (10) : 757 - 766
[10] The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores
Fischer, T
Strässer, K
Rácz, A
Rodriguez-Navarro, S
Oppizzi, M
Ihrig, P
Lechner, J
Hurt, E
[J]. EMBO JOURNAL, 2002, 21 (21) : 5843 - 5852

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