Assessment of genetic diversity within and among germplasm accessions in cultivated sorghum using microsatellite markers

被引：98

作者：

Djé, Y

Heuertz, M

Lefèbvre, C

Vekemans, X

机构：

[1] Free Univ Brussels, Lab Genet & Ecol Vegetales, B-1160 Brussels, Belgium

[2] Ctr Rech Publ, CREBS, Res Unit, L-1511 Luxembourg, Luxembourg

来源：

THEORETICAL AND APPLIED GENETICS | 2000年 / 100卷 / 06期

关键词：

core collection; genetic diversity; germplasm; microsatellites; sorghum; SSR;

D O I：

10.1007/s001220051371

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Microsatellite markers are increasingly being used in crop plants to discriminate among genotypes and as tools in marker-assisted selection. Here we evaluated the use of microsatellite markers to quantify the genetic diversity within as well as among accessions sampled from the world germplasm collection of sorghum. Considerable variation was found at the five microsatellite loci analysed, with an average number of alleles per locus equal to 2.4 within accessions and 19.2 in the overall sample of 25 accessions. The collection of sorghum appeared highly structured genetically with about 70% of the total genetic diversity occurring among accessions. However, differentiation among morphologically defined races of sorghum, or among geographic origins, accounted for less than 15% of the total genetic diversity. Our results are in global agreement with those obtained previously with allozyme markers. We were also able to show that microsatellite data are useful in identifying individual accessions with a high relative contribution to the overall allelic diversity of the collection.

引用

页码：918 / 925

页数：8

共 45 条

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