Effect of periodontitis and smoking on blood leukocytes and acute-phase proteins

被引:117
作者
Fredriksson, MI
Figueredo, CMS
Gustafsson, A
Bergström, KG
Åsman, BE
机构
[1] Huddinge Univ Hosp, Karolinska Inst, Dept Med Lab Sci & Technol, Div Clin Chem, S-14186 Huddinge, Sweden
[2] Karolinska Inst, Inst Odontol, Div Periodontol, Stockholm, Sweden
关键词
periodontitis/complications; smoking/adverse effects; neutrophils; controlled clinical trials;
D O I
10.1902/jop.1999.70.11.1355
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Background: We have previously found hyperreactive neutrophils, intrinsic or induced, in periodontitis patients by in vitro quantitation of free oxygen radicals. The effects of periodontitis and cigarette smoking on blood parameters have generally been described separately. Our aim was to compare these systemic effects of periodontitis and cigarette smoking, separately and in combination, in order to study the hyperreactivity in peripheral neutrophils. Methods: Blood cells and acute-phase proteins were studied in 40 periodontitis patients and 43 healthy controls. The generation of free oxygen radicals from neutrophils was measured as luminol-enhanced chemiluminescence (CL) after activation of their Fc gamma receptors with opsonized Staphylococcus aureus. Results: An increase in CL in peripheral neutrophils from periodontitis patients was confirmed. Smoking had no significant effect on CL. The periodontitis patients had higher concentrations of C-reactive protein (CRP) than the controls. ANOVA analysis showed that the increase in neutrophil count, haptoglobin, and alpha-1 antitrypsin levels was significantly influenced by cigarette smoking. IgG(2) was lower in patients than in controls (P <0.017, ANOVA), and there was an interaction between periodontitis and smoking (P <0.047, ANOVA). The lower concentration of IgG(2) in patients who smoke may impair neutrophil function and be a mechanism by which smoking aggravates periodontitis. Conclusions: In general, the combination of periodontitis and cigarette smoking alters the parameters studied. The effects of periodontitis on CRP and IgG(2) means that periodontal lesions may also leak agents, priming the peripheral neutrophils to increased CL.
引用
收藏
页码:1355 / 1360
页数:6
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