Removal of humic substances from soil DNA using aluminium sulfate

被引：76

作者：

Dong, Dexian ^{[1
]}

Yan, An ^{[1
]}

Liu, Haiming ^{[1
]}

Zhang, Xuehong ^{[1
]}

Xu, Yuquan ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Lab Mol Microbiol, Coll Life Sci & Biotechnol, Shanghai 200240, Peoples R China

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 66卷 / 02期

基金：

中国国家自然科学基金;

关键词：

DNA extraction; humic substance; aluminium sulfate; extracellular DNA;

D O I：

10.1016/j.mimet.2005.11.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct extraction of soil DNA has become essential for the study of soil microorganisms. Humic substances co-extracted during DNA retrieval is a big problem because it greatly inhibits the enzymes involved in manipulating DNA. Popular commercial kits available for soil DNA extraction are unable to overcome this problem. Here we report an effective protocol for the removal of humic substance from soil DNA. The protocol involves flocculation of the humic substance by excessive Al3+, then removal of superfluous Al3+ via pH adjustment and finally release of soil microbial DNA by SDS lysis. This technique is superior to that employed by the UltraClean Soil DNA Kit and can be applied to a wide variety of soils. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：217 / 222

页数：6

共 26 条

[1]

[Anonymous], 1990, PCR PROTOCOLS, DOI DOI 10.1016/B978-0-12-372180-8.50042-1

[2] PREFERENTIAL UPTAKE OF FE(III) BY HUMIC-ACID EXTRACTED FROM LIGNITE [J].