Liquid chromatography-tandem mass spectrometry of some anabolic steroids

被引：51

作者：

Joos, PE ^{[1
]}

Van Ryckeghem, M ^{[1
]}

机构：

[1] SGS Depauw & Stokoe, Dept IAC, B-9120 Melsele, Belgium

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 20期

关键词：

D O I：

10.1021/ac981073s

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper a procedure is described for the analysis of 36 anabolic steroids, regularly found in kidney fat matrixes. After preparative HPLC is carried out, six fractions, containing:the different steroids, were obtained. These fractions were then separated and on-line mass-analyzed, using APcI LC/MS. When used in tandem mass spectrometry (MRM) mode, it is possible to obtain detection limits below l-ppb for all steroids and below 0.1 ppb for most of them, As such, it is possible to meet the regulations for detection of these anabolic steroids. To verify the influence of matrixes, real-world samples were analyzed in the same manner. This resulted in almost analog detection limits.

引用

页码：4701 / 4710

页数：10

共 20 条

[1] DEVELOPMENTS IN RESIDUE ASSAY AND METABOLISM STUDY OF GROWTH-PROMOTERS BY MASS-SPECTROMETRIC ANALYSIS [J].