Pulsed-Field Gel Electrophoresis characterization of Listeria monocytogenes isolates from cheese manufacturing plants in Sao Paulo, Brazil

被引：48

作者：

Barancelli, Giovana V. ^{[1
]}

Camargo, Tarsila M. ^{[2
]}

Gagliardi, Natalia G. ^{[1
]}

Porto, Ernani ^{[2
]}

Souza, Roberto A. ^{[3
]}

Campioni, Fabio ^{[3
]}

Falcao, Juliana P. ^{[3
]}

Hofer, Ernesto ^{[4
]}

Cruz, Adriano G. ^{[5
]}

Oliveira, Carlos A. F. ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Engn Alimentos, BR-13635900 Pirassununga, SP, Brazil

[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Agroind Alimentos & Nutr, Piracicaba, SP, Brazil

[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14049 Ribeirao Preto, SP, Brazil

[4] Inst Oswaldo Cruz, Lab Zoonoses Bacterianas, Rio De Janeiro, RJ, Brazil

[5] Inst Fed Educ Ciencia & Tecnol Rio de Janeiro, Rio De Janeiro, RJ, Brazil

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2014年 / 173卷

基金：

巴西圣保罗研究基金会;

关键词：

L; monocytogenes; Cheese; Environment; PFGE; MINAS-FRESCAL CHEESE; MOLECULAR EPIDEMIOLOGY; UNITED-STATES; PFGE ANALYSIS; RAW-MILK; PREVALENCE; SPP; ENVIRONMENT; STRAINS; QUALITY;

D O I：

10.1016/j.ijfoodmicro.2013.12.018

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

This study aimed to evaluate the occurrence of Listeria monocytogenes in cheese and in the environment of three small-scale dairy plants (A, B, C) located in the Northern region state of Sao Paulo, Brazil, and to characterize the isolates using conventional serotyping and PFGE. A total of 393 samples were collected and analyzed from October 2008 to September 2009. From these, 136 came from dairy plant A, where only L. seeligeri was isolated. In dairy plant B, 136 samples were analyzed, and L. innocua, L. seeligeri and L. welshimeri were isolated together with L. monocytogenes. In dairy plant C, 121 samples were analyzed, and L. monocytogenes and L. innocua were isolated. Cheese from dairy plants B and C were contaminated with Listeria spp, with L. innocua being found in Minas frescal cheese from both dairy plants, and L. innocua and L. monocytogenes in Prato cheese from dairy plant C. A total of 85 L. monocytogenes isolates were classified in 3 serotypes: 1/2b, 1/2c, and 4b, with predominance of serotype 4b in both dairy plants. The 85 isolates found in the dairy plants were characterized by genomic macrorestriction using ApaI and AscI with Pulsed Field Gel Electrophoresis (PFGE). Macrorestricdon yielded 30 different pulsotypes. The presence of indistinguishable profiles repeatedly isolated during a 12-month period indicated the persistence of L. monocytogenes in dairy plants B and C, which were more than 100 km away from each other. Brine used in dairy plant C contained more than one L. monocytogenes lineage. The routes of contamination were identified in plants B and C, and highlighted the importance of using molecular techniques and serotyping to track L. monocytogenes sources of contamination, distribution, and routes of contamination in dairy plants, and to develop improved control strategies for L. monocytogenes in dairy plants and dairy products. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：21 / 29

页数：9

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