Expression of Rsf-1, a chromatin-remodeling gene, in ovarian and breast carcinoma

被引:35
作者
Mao, Tsui-Lien
Hsu, Chih-Yi
Yen, May J.
Gilks, Blake
Sheu, Jim Jinn-Chyuan
Gabrielson, Edward
Vang, Russell
Cope, Leslie
Kurman, Robert J.
Wang, Tian-Li
Shih, Ie-Ming [1 ]
机构
[1] Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21231 USA
[2] Johns Hopkins Med Inst, Dept Oncol, Baltimore, MD 21231 USA
[3] Johns Hopkins Med Inst, Dept Gynecol, Baltimore, MD 21231 USA
[4] Vancouver Gen Hosp, Dept Pathol, Vancouver, BC V5E 1M9, Canada
[5] Natl Taiwan Univ Hosp, Dept Pathol, Taipei 10001, Taiwan
[6] Taipei Vet Gen Hosp, Dept Pathol, Taipei 11221, Taiwan
[7] Natl Yang Ming Univ, Sch Med, Taipei 11221, Taiwan
关键词
Rsf-1; ovarian serous carcinoma; breast carcinoma;
D O I
10.1016/j.humpath.2006.04.008
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Rsf-1 protein is a member of a chromatin-remodeling complex that plays an important role in regulating gene expression and cell proliferation. Our previous study showed that Rsf-1 was an amplified gene that participated in the development of ovarian serous carcinoma. To further elucidate the role of Rsf-1 in ovarian cancer, we studied Rsf-1 immunoreactivity in 294 ovarian tumors of various histologic types. Because the Rsf-1 amplicon overlaps an amplified region reported in breast cancer, we included 782 neoplastic and normal breast tissues for comparison. Immunohistochemistry was performed on tissue microarrays using a 4-tiered scoring system. Overexpression of Rsf-1 was defined as a nuclear immunointensity of 3+ to 4+ because of a strong correlation between 3+ and 4+ immunointensity and Rsf-1 gene amplification, based on our previous fluorescence in situ hybridization analysis. Rsf-1 overexpression was observed in 25% of high-grade ovarian serous carcinomas and in only rare cases (< 7%) of low-grade ovarian serous, ovarian endometrioid, and invasive breast carcinomas but not in any ovarian serous borderline tumors, ovarian clear cell carcinomas, ovarian mucinous carcinomas, intraductal carcinomas of the breast, and normal ovaries and breast tissues. Thus, overexpression of Rsf-1 was significantly associated with high-grade ovarian serous carcinoma (P < .05), as compared with other types of ovarian tumors and breast carcinomas. Our results provide evidence that Rsf-1 expression is primarily confined to high-grade serous carcinoma, the most aggressive ovarian cancer. Because Rsf-1 overexpression occurs in only a small number of breast carcinomas, it is unlikely that Rsf-1 is a critical gene in the development of breast carcinoma. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:1169 / 1175
页数:7
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