DNA nucleic acid sequence-based amplification-based genotyping for polymorphism analysis

被引：12

作者：

Berard, C ^{[1
]}

Cazalis, MA ^{[1
]}

Leissner, P ^{[1
]}

Mougin, B ^{[1
]}

机构：

[1] BioMerieux, Human Genet Dept, F-69280 Marcy Letoile, France

来源：

BIOTECHNIQUES | 2004年 / 37卷 / 04期

关键词：

D O I：

10.2144/04374DD04

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleic acid sequence-based amplification (NASBA) is a sensitive isothermal transcription-based amplification method known to be a suitable tool for RNA research. We demonstrate that NASBA technology can be applied to single nucleotide polymorphism (SNP) analysis using human genomic DNA as a template. Combination of DNA NASBA with multiplex hybridization of specific molecular beacons makes it possible to unambiguously discriminate the presence of the SNP of interest. This protocol is easy-to-use, robust, and makes it possible to rapidly detect single nucleotide substitutions in clinical or cell line DNA sequences using a large range of DNA input. Such a real-time genotyping DNA NASBA assay can find broad application in clinical diagnostics.

引用

页码：680 / +

页数：5

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