Virosomes in vaccine development: Induction of cytotoxic T lymphocyte activity with virosome-encapsulated protein antigens

被引:17
作者
Bungener, L [1 ]
Idema, J [1 ]
ter Veer, W [1 ]
Huckriede, A [1 ]
Daemen, T [1 ]
Wilschut, J [1 ]
机构
[1] Univ Groningen, Dept Med Microbiol, Mol Virol Sect, NL-9713 AV Groningen, Netherlands
关键词
virosome; cytotoxic T lymphocyte; CTL; antigen presentation; dendritic cells; antigen-presenting cells; xvaccine design;
D O I
10.1081/LPR-120004789
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Virosomes are reconstituted viral envelopes which lack the genetic material but retain the cell entry and membrane fusion characteristics of the virus they are derived from. Thus, influenza virosomes are taken up by cells via receptor-mediated endocytosis, which directs the particles to the endosomal cell compartment. Subsequently, the virosomal membrane fuses with the endosomal membrane induced by the mildly acidic pH within the endosomes. This fusion process establishes continuity between the lumen of the virosome and the cell cytosol. Upon interaction of virosomes with antigen-presenting cells (APCs), protein antigens encapsulated within virosomes will be delivered to the cell cytosol, and thus, into the MHC class I presentation pathway. Indeed, virosome-mediated delivery of antigens in vivo results in efficient priming of a class I MHC-restricted cytotoxic T lymphocyte (CTL) response.
引用
收藏
页码:155 / 163
页数:9
相关论文
共 15 条
  • [1] Induction of cytotoxic T lymphocyte activity by fusion-active peptide-containing virosomes
    Arkema, A
    Huckriede, A
    Schoen, P
    Wilschut, J
    Daemen, T
    [J]. VACCINE, 2000, 18 (14) : 1327 - 1333
  • [2] CELLULAR CYTOPLASMIC DELIVERY OF A POLYPEPTIDE TOXIN BY RECONSTITUTED INFLUENZA-VIRUS ENVELOPES (VIROSOMES)
    BRON, R
    ORTIZ, A
    WILSCHUT, J
    [J]. BIOCHEMISTRY, 1994, 33 (31) : 9110 - 9117
  • [3] PREPARATION, PROPERTIES, AND APPLICATIONS OF RECONSTITUTED INFLUENZA-VIRUS ENVELOPES (VIROSOMES)
    BRON, R
    ORTIZ, A
    DIJKSTRA, J
    STEGMANN, T
    WILSCHUT, J
    [J]. METHODS IN ENZYMOLOGY, 1993, 220 : 313 - 331
  • [4] BUNGENER LB, 2002, IN PRESS VACCINE
  • [5] Virosomes as an antigen delivery system
    Daemen, T
    Bungener, L
    Huckriede, A
    Wilschut, J
    [J]. JOURNAL OF LIPOSOME RESEARCH, 2000, 10 (04) : 329 - 338
  • [6] Daemen T, 1998, MEDICAL APPLICATIONS OF LIPOSOMES, P117, DOI 10.1016/B978-044482917-7/50008-9
  • [7] Virus maturation by budding
    Garoff, H
    Hewson, R
    Opstelten, DJE
    [J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (04) : 1171 - +
  • [8] Virus-cell and cell-cell fusion
    Hernandez, LD
    Hoffman, LR
    Wolfsberg, TG
    White, JM
    [J]. ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1996, 12 : 627 - 661
  • [9] HUCKRIEDE A, 2002, IN PRESS METH ENZYMO
  • [10] Gene transfer mediated by fusion protein hemagglutinin reconstituted in cationic lipid vesicles
    Schoen, P
    Chonn, A
    Cullis, PR
    Wilschut, J
    Scherrer, P
    [J]. GENE THERAPY, 1999, 6 (05) : 823 - 832