Dark pretreatment improves adventitious shoot organogenesis from cotyledons of diploid watermelon

被引：34

作者：

Compton, ME ^{[1
]}

机构：

[1] Univ Wisconsin Platteville, Sch Agr, Platteville, WI 53818 USA

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1999年 / 58卷 / 03期

关键词：

caulogenesis; Citrullus lanatus; Cucurbitaceae; photoperiod;

D O I：

10.1023/A:1006364013126

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The influence of light incubation during embryo germination on shoot organogenesis from cotyledons of four diploid watermelon [Citrullus lanatus (Thumb.) Matsum. & Nakai] cultivars was examined. Germinating embryos in darkness significantly improved the number of explants that produced harvestable shoots during the 6 week incubation period on shoot regeneration medium under a 16-h photoperiod. The percentage of explants with shoots more than doubled for 'Crimson Sweet' and was about 1.5-fold greater for 'Sweet Gem' and 'Yellow Doll' when embryos were germinated in darkness. The percentage of explants with shoots was not significantly improved for 'Minilee' by pretreating seedlings in darkness. This study demonstrates that optimal shoot regeneration can be obtained by germinating embryos in darkness before preparing cotyledon explants.

引用

页码：185 / 188

页数：4

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