Circular dichroism and conformational polymorphism of DNA

被引：1479

作者：

Kypr, Jaroslav ^{[1
]}

Kejnovska, Iva ^{[1
]}

Renciuk, Daniel ^{[1
]}

Vorlickova, Michaela ^{[1
]}

机构：

[1] Acad Sci Czech Republic, Inst Biophys, CZ-61265 Brno, Czech Republic

来源：

NUCLEIC ACIDS RESEARCH | 2009年 / 37卷 / 06期

关键词：

GUANINE-GUANINE STACKING; HUMAN TELOMERIC SEQUENCE; ADENINE REPEAT STRANDS; DOUBLE-HELICAL DNA; G-QUADRUPLEX; NUCLEIC-ACID; POTASSIUM SOLUTION; CRYSTAL-STRUCTURE; MELTING CURVES; SYNTHETIC DNA;

D O I：

10.1093/nar/gkp026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we review studies that provided important information about conformational properties of DNA using circular dichroic (CD) spectroscopy. The conformational properties include the B-family of structures, A-form, Z-form, guanine quadruplexes, cytosine quadruplexes, triplexes and other less characterized structures. CD spectroscopy is extremely sensitive and relatively inexpensive. This fast and simple method can be used at low- as well as high-DNA concentrations and with short- as well as long-DNA molecules. The samples can easily be titrated with various agents to cause conformational isomerizations of DNA. The course of detected CD spectral changes makes possible to distinguish between gradual changes within a single DNA conformation and cooperative isomerizations between discrete structural states. It enables measuring kinetics of the appearance of particular conformers and determination of their thermodynamic parameters. In careful hands, CD spectroscopy is a valuable tool for mapping conformational properties of particular DNA molecules. Due to its numerous advantages, CD spectroscopy significantly participated in all basic conformational findings on DNA.

引用

页码：1713 / 1725

页数：13

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