A polygalacturonase of animal origin isolated from the root-knot nematode Meloidogyne incognita

被引:108
作者
Jaubert, S [1 ]
Laffaire, JB [1 ]
Abad, P [1 ]
Rosso, MN [1 ]
机构
[1] INRA, Unite Interact Plantes Microorganismes & Sante Ve, F-60606 Antibes, France
关键词
nematode; pectin; polygalacturonase; stylet secretion;
D O I
10.1016/S0014-5793(02)02906-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The first animal polygalacturonase (PG, EC 2.1.15) encoding cDNA, Mi-pg-1, was cloned from the plant parasitic nematode Meloidogyne incognita. The enzymatic activity, of MI-PG-1 was confirmed after heterologous expression in Escherichia coli. The presence of a predicted signal peptide on the MI-PG-1 sequence together with the specific localization of the transcripts of the Mi-pg-1 gene in the oesophageal glands of infective juveniles imply that MI-PG-1 could be secreted into plant tissues. The potential role of MI-PG-1 in parasitism is discussed. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:109 / 112
页数:4
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