Ligand-directed tosyl chemistry for protein labeling in vivo

被引：292

作者：

Tsukiji, Shinya ^{[1
]}

Miyagawa, Masayoshi ^{[1
]}

Takaoka, Yousuke ^{[1
]}

Tamura, Tomonori ^{[1
]}

Hamachi, Itaru ^{[1
,2
]}

机构：

[1] Kyoto Univ, Grad Sch Engn, Dept Synthet Chem & Biol Chem, Nishikyo Ku, Kyoto, Japan

[2] Japan Sci & Technol Agcy, CREST, Chiyoda Ku, Tokyo, Japan

来源：

NATURE CHEMICAL BIOLOGY | 2009年 / 5卷 / 05期

基金：

日本学术振兴会;

关键词：

CARBONIC-ANHYDRASE INHIBITORS; UNNATURAL AMINO-ACIDS; ISOZYME-IX; REACTIVITY; SURFACE; PROBES; DESIGN; CELLS; F-19;

D O I：

10.1038/nchembio.157

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we describe a method for the site-selective attachment of synthetic molecules into specific 'endogenous' proteins in vivo using ligand-directed tosyl (LDT) chemistry. This approach was applied not only for chemically labeling proteins in living cells, tissues and mice but also for constructing a biosensor directly inside cells without genetic engineering. These data establish LDT chemistry as a new tool for the study and manipulation of biological systems.

引用

页码：341 / 343

页数：3

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