Milsana-induced resistance in powdery mildew-infected cucumber plants correlates with the induction of chalcone synthase and chalcone isomerase

被引：79

作者：

Fofana, B

McNally, DJ

Labbé, C

Boulanger, R

Benhamou, N

Séguin, A

Bélanger, RR

机构：

[1] Univ Laval, Dept Phytol, Lab Biocontrole, Ste Foy, PQ G1K 7P4, Canada

[2] Univ Laval, Dept Phytol Rech Sci Vie & Sante, Ste Foy, PQ G1K 7P4, Canada

[3] Canadian Forest Serv, Nat Resources Canada, Laurentian Forestry Ctr, Ste Foy, PQ G1V 4C7, Canada

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2002年 / 61卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

CHS; CHI; IFR; Milsana; induced resistance; flavonoid phytoalexins; Cucumis sativus; Sphaerotheca fuliginea; Powdery mildew; Reynoutria sachalinensis; Medicago sativa; Escherichia coli;

D O I：

10.1016/S0885-5765(02)90420-0

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cucumber plants (Cucumis sativus cv Corona) infected with powdery mildew (Sphaerotheca fuliginea) were used to study molecular and biochemical changes occurring in leaves following treatments with Milsana(R), a reported inducer of defence reactions in cucumber. Plants treated with Milsana(R) were significantly less infected than controls and this protective effect against powdery mildew was maintained over time. Based on analyses of foliar tissues, this induced resistance correlated with an increased extractable enzymatic activity and mRNA accumulation of two flavonoid biosynthetic genes, chalcone synthase (CHS) and chalcone isomerase (CHI) and the accumulation of flavonoid compounds in treated plants. These results are the first to link CHS and CHI increased activities with induced resistance in cucumber and highlight the implication of flavonoids in the complex defence system of this plant species. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：121 / 132

页数：12

共 61 条

[1] Cloning and functional expression of a cytochrome P450 cDNA encoding 2-hydroxyisoflavanone synthase involved in biosynthesis of the isoflavonoid skeleton in licorice [J].