Specific identification of the enteropathogens Campylobacter jejuni and Campylobacter coli by using a PCR test based on the ceuE gene encoding a putative virulence determinant

被引：218

作者：

Gonzalez, I ^{[1
]}

Grant, KA ^{[1
]}

Richardson, PT ^{[1
]}

Park, SF ^{[1
]}

Collins, MD ^{[1
]}

机构：

[1] INST FOOD RES, READING LAB, BBSRC, READING RG6 6BZ, BERKS, ENGLAND

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 03期

关键词：

D O I：

10.1128/JCM.35.3.759-763.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A PCR method for the rapid identification and discrimination of thermophilic Campylobacter jejuni and Campylobacter coli was developed by using a gene encoding a protein involved in siderophore transport (ceuE). A nucleotide sequence divergence of approximately 13% in the ceuE genes of C. jejuni and C. coli facilitated the design of two species-specific PCR primer sets. The specificity of the PCR amplification reactions was confirmed by using two nonradioactively labelled species-specific internal oligonucleotide hybridization probes for each of these species.

引用

页码：759 / 763

页数：5

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