3D structural information as a guide to protein engineering using genetic selection

被引：33

作者：

Kast, P ^{[1
]}

Hilvert, D ^{[1
]}

机构：

[1] Scripps Res Inst, DEPT CHEM, LA JOLLA, CA 92037 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1997年 / 7卷 / 04期

关键词：

D O I：

10.1016/S0959-440X(97)80109-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A great variety of protein systems have been investigated in the past year using structure-guided evolutionary strategies. On the basis of available 3D structural information, critical regions of proteins have been targeted for randomizing mutagenesis and active variants of the corresponding genes have been selected. These approaches help characterize structural and mechanistic features of proteins and have important implications far design.

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页码：470 / 479

页数：10

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