Agrobacterium tumefaciens-mediated transformation of Robinia pseudoacacia

被引:47
作者
Igasaki, T
Mohri, T
Ichikawa, H
Shinohara, K
机构
[1] Forestry & Forest Prod Res Inst, Bioresources Technol Div, Mol & Cell Biol Sect, Ibaraki, Osaka 3058687, Japan
[2] Natl Inst Agrobiol Resources, Dept Biotechnol, Tsukuba, Ibaraki 3058602, Japan
基金
日本科学技术振兴机构;
关键词
Agrobacterium tumefaciens; hygromycin phosphotransferase; regeneration; Robinia pseudoacacia; transformation;
D O I
10.1007/s002990050754
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Genetically transformed Robinia pseudoacacia (black locust) plants were regenerated after co-cultivation of stem and leaf segments with Agrobacterium tumefaciens strain GV3101 (pMP90) that harbored a binary vector that included genes for beta-glucuronidase (GUS) and hygromycin phosphotransferase. Successful transformation was confirmed by the ability of stem and leaf segments to produce calli in the presence of hygromycin, by histochemical and fluorometric assays of GUS activity in plant tissues, and by Southern blotting analysis. In this transformation system, about 2 months were required for regeneration of transgenic plants from stem and leaf segments. The frequency of transformation from stem segments was approximately 24%, and the morphology of regenerated plants resembled that of the original parental strain.
引用
收藏
页码:448 / 453
页数:6
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